Background: Bacopa monnieri Linn. (Plantaginaceae), a well-known medicinal plant, is widely used in traditional\nmedicine system. It has long been used in gastrointestinal discomfort, skin diseases, epilepsy and analgesia. This\nresearch investigated the in vitro antimicrobial activity of Bacopa monnieri leaf extract against Staphylococcus aureus\nand the interaction of possible compounds involved in this antimicrobial action.\nMethods: Non-edible plant parts were extracted with ethanol and evaporated in vacuo to obtain the crude extract.\nA zone of inhibition studies and the minimum inhibitory concentration (MIC) of plant extracts were evaluated\nagainst clinical isolates by the microbroth dilution method. Docking study was performed to analyze and identify\nthe interactions of possible antimicrobial compounds of Bacopa monnieri in the active site of penicillin binding\nprotein and DNA gyrase through GOLD 4.12 software.\nResults: A zone of inhibition studies showed significant (p < 0.05) inhibition capacity of different concentrations of\nBacopa monnieri�s extract against Staphylococcus aureus. The extract also displayed very remarkable minimum\ninhibitory concentrations (?16 ?g/ml) which was significant compared to that (?75 ?g/ml) of the reference\nantibiotic against the experimental strain Staphylococcus aureus. Docking studies recommended that luteolin, an\nexisting phytochemical of Bacopa monnieri, has the highest fitness score and more specificity towards the DNA\ngyrase binding site rather than penicillin binding protein.\nConclusions: Bacopa monnieri extract and its compound luteolin have a significant antimicrobial activity against\nStaphylococcus aureus. Molecular binding interaction of an in silico data demonstrated that luteolin has more\nspecificity towards the DNA gyrase binding site and could be a potent antimicrobial compound.
Loading....